Isolation and structural elucidation of AC326-alpha, a new member of the moenomycin group.

(0.25 mg/liter), and CaCO3(7 g/liter). Air was sparged at 10 liter/minute; agitation was set at 500 rpm; temperature was maintained at 28°C; dissolved oxygen was set to 99.9% at 0 time. The whole mash was harvested after it was incubated for 4 days. The pellet obtained by centrifugation was extracted with methanol (6 liters) and the extract, upon concentration to a volume of 1 liter, was partitioned between dichloromethane and water (3 liters for both layers). The aqueous layer was pp.191 195